ABSTRACT
Objective To evaluate the association of the SYK gene promoter-803A T polymorphism and breast cancer sus ceptibility during Han descent female population in Beijing area.Methods In this case control study,genotype of SYK (A＞T at-803,rs290987) was determined by polymerase chain reaction combined with DNA direct sequencing in blood samples of 57 breast cancer cases and 60 age matched bealthy controls.They were all female.Genotype and allele frequency distribu tions of 803A T were determined and analyzed.ELISA test was performed to detect the serum level of SYK in the two groups.Results The frequencies of the T allele and TA+TT genotypes of the 803A＞T were found to be significantly higher in the breast cancer patients in contrast to the healthy individuals of the control group(x2 =5.348,P=0.021).Pa tients with the T allele (TA+TT) had a 2.87-fold risk of developing breast cancer compared with those with the A allele (AA) (OR=2.87;95％ CI=1.27～6.49).The test group had significantly decreased level of SYK in serum compared to normal controls (F 33.278,P＜0.01).Conclusion This study revealed that the genotype TA and TT of the SYK gene-803 loci of Han female population in the Beijing area are the risk factors for the breast cancer.
ABSTRACT
Objective@#To obtain recombinant major surface glycoprotein(rMsg)of Pneumocystis jirovecii(Pj) and survey anti-Pj rMsg IgG and IgM antibodies using enzyme-linked immunosorbent assay (ELISA) among randomly selected sera from Beijing local children under 14 years of age.@*Methods@#ELISA method were established to detect anti-Pj rMsg IgG and IgM antibodies. We randomly selected sera of 201 cases who were under 14 years of age were screened.@*Results@#There were 78 cases in the age group of 1 day to 30 days, 33 cases in the group of 1 month to 12 months, and 90 cases in the group of 1 year to 14 years. The positive rates of anti-Pj rMsg IgG antibody in these three age groups were 10.3%(8/78), 21.2%(7/33), and 41.1%(37/90)respectively(χ2=21.190, P=0.000). The positive rates of anti-Pj rMsg IgM antibody were 6.4%(5/78), 39.4%(13/33), and 76.7%(69/90)respectively(χ2=84.260, P=0.000). The overall positive rates of anti-Pj rMsg IgG and IgM antibodies were 25.9%(52/201)and 43.3%(87/201)respectively(χ2=53.463, P=0.000). Among these, simultaneously IgG positive IgM positive, IgG positive IgM negative, IgG negative IgM positive and IgG negative IgM negative rates were 22.4%(45/201), 3.5%(7/201), 20.9%(42/201), and 53.2%(107/201)respectively.@*Conclusions@#The overall serological response rate after Pj exposure, either anti-Pj IgG or IgM antibody positive, was as high as 46.8% in children under 14 years of age in Beijing area. The positive rates of anti-rMsg IgG and IgM antibodies increased with age, with highest rate in age group of more than one years old.